Fraxin; akut mezenter iskemisinin farmakolojik tedavisinde umut veren molek�l: Deneysel bir �al��ma

AYDIN, ISMAIL; UYGUR, Furkan Ali; EMECEN, OMER; Sengul, Demet

Cilt : 31 Say� : 12 Y�l : 2025

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Fraxin; akut mezenter iskemisinin farmakolojik tedavisinde umut veren molek�l: Deneysel bir �al��ma [Ulus Travma Acil Cerrahi Derg]

Ulus Travma Acil Cerrahi Derg. Bask�daki Makaleler: UTD-21860

Fraxin; akut mezenter iskemisinin farmakolojik tedavisinde umut veren molek�l: Deneysel bir �al��ma

ISMAIL AYDIN¹, Furkan Ali UYGUR¹, OMER EMECEN², Demet Sengul³
¹Giresun �niversitesi T�p Fak�ltesi, Genel Cerrahi Ana Bilim Dal�, Giresun
²Giresun �niversitesi T�p Fak�ltesi, Biyokimya Ana Bilim Dal�, Giresun
³Giresun �niversitesi T�p Fak�ltesi, Patoloji Ana Bilim Dal�, Giresun

Ama�: Akut mezenter iskemi (AMI), ince ba��rsa�a kan ak��n�n ani kesilmesi sonucu olu�an, ba��rsak nekrozu ve kar�n a�r�s�n�n nadir nedenlerinden biridir. Tan� ve tedavideki gecikme mortalitede ciddi art��lara neden olmaktad�r. Bu �al��mada antiinflamatuar ve antioksidan etkileri oldu�u bilinen Fraxin'in ba��rsak iskemi-repurf�zyon hasar� �zerindeki etkilerinin ara�t�r�lmas� ama�land�.

Y�ntemler: Bu �al��ma, sa�l�kl� erkek Wistar Albino s��anlar kullan�larak kontroll� deneysel tasar�mda ger�ekle�tirildi. S��anlar d�rt gruba ayr�ld�: Sham grubu (SMA izole edilmi� ancak kapat�lmam��), Kontrol grubu (SMA izole edilmi� ve I-R ile ind�klenmi�), 10 mg/kg Fraxin grubu ve 50 mg/kg Fraxin grubu (reperf�zyondan �nce Fraxin uyguland�). Toplam antioksidan kapasite (TAS), toplam oksidan durum (TOS), s�peroksit dismutaz (SOD), glutatyon peroksidaz (GPx) ve katalaz (CAT) aktiviteleri de�erlendirildi. Histopatolojik incelemeler ve inflamatuar belirte�ler (TNF-α, IL-6 ve MPO) da analiz edildi.
Bulgular: Sham grubunda SOD aktivitesi 135,2 � 10,5 U/mg protein, GPx aktivitesi 65,3 � 4,7 U/mg protein ve CAT aktivitesi 85,1 � 5,8 U/mg protein olarak belirlendi. Kontrol grubunda ise bu de�erler s�ras�yla 95,4�7,9, 45,7�3,6 ve 60,3�4,2 U/mg protein olarak belirlendi. 10 mg/kg Fraxin grubunda SOD 115,6�8,4, GPx 55,8�4,2 ve CAT 75,6�5,5 U/mg protein; 50 mg/kg Fraxin grubunda SOD 130,8 � 9,7, GPx 60,2 � 4,8 ve CAT 90,4 � 6,3 U/mg protein. Fraxin uygulanan gruplarda TNF-α, IL-6 ve MPO d�zeylerinde anlaml� d��ler g�zlendi (p<0,05).
Sonu�: Fraxin'in mezenterik iskemi-repurf�zyon hasar�nda dokular� korumas�, inflamasyonu ve oksidatif stresi azaltarak antioksidan g�stergeleri g��lendirmesi nedeniyle bu hastal��n farmakolojik tedavisinde potansiyel bir ajan olarak kullan�labilece�inin ak�lda tutulmas� gerekti�ini d��n�yoruz.

Anahtar Kelimeler: mezenter iskemi, iskemi-reperf�zyon hasar�, fraxin, antioksidan aktivite, antiinflamatuar aktivite, koruyucu etki

Fraxin; acute mesenteric ischemia promising molecule in pharmacological treatment: An experimental study

ISMAIL AYDIN¹, Furkan Ali UYGUR¹, OMER EMECEN², Demet Sengul³
¹Department Of General Surgery, Giresun University, Giresun, Turkey
²Department Of Biochemistry, Giresun University, Giresun, Turkey
³Department Of Pathology, Giresun University, Giresun, Turkey

Background: Acute mesenteric ischemia (AMI) is one of the rare causes of intestinal necrosis and abdominal pain caused by sudden cessation of blood flow to the small intestine. Delay in diagnosis and treatment causes serious increases in mortality. This study aimed to investigate the effects of Fraxin, which is known to have anti-inflammatory and antioxidant effects, on intestinal ischemia-repurfusion injury.

Methods: This study was conducted in a controlled experimental design using healthy male Wistar Albino rats. The rats were divided into four groups: Sham group (SMA isolated but not occluded), Control group (SMA isolated and I-R induced), 10 mg/kg Fraxin group and 50 mg/kg Fraxin group (Fraxin administered before reperfusion). Total antioxidant capacity (TAS), total oxidant status (TOS), superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) activities were evaluated. Histopathologic examinations and inflammatory markers (TNF-α, IL-6 and MPO) were also analyzed.
Results: In the sham group, SOD activity was 135.2 � 10.5 U/mg protein, GPx activity was 65.3 � 4.7 U/mg protein and CAT activity was 85.1 � 5.8 U/mg protein. In the control group, these values were 95.4 � 7.9, 45.7 � 3.6 and 60.3 � 4.2 U/mg protein, respectively. In 10 mg/kg Fraxin group, SOD 115.6 � 8.4, GPx 55.8 � 4.2 and CAT 75.6 � 5.5 U/mg protein; in 50 mg/kg Fraxin group, SOD 130.8 � 9.7, GPx 60.2 � 4.8 and CAT 90.4 � 6.3 U/mg protein. Significant decreases were observed in TNF-α, IL-6 and MPO levels in Fraxin treated groups (p<0.05).
Conclusions: We think that it should be kept in mind that Fraxin can be used as a potential agent in the pharmacological treatment of this disease because it protects tissues in mesenteric ischemia-repurfusion injury and strengthens antioxidant indicators by reducing inflammation and oxidative stress.

Keywords: mesenteric ischemia, ischemia-reperfusion injury, fraxin, antioxidant activity, anti-inflammatory activity, protective effect

Sorumlu Yazar: ISMAIL AYDIN, T�rkiye
Makale Dili: �ngilizce

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