Ulus Travma Acil Cerrahi Derg. Ahead of Print: UTD-52279

Effects of Epigallocatechin Gallate on Ischemia-reperfusion Injury: An Experimental Study in Rats

Yusuf Ergün¹, Yasarcan Baykisi², Metin Kilinc², AYÞE NUR MAVÝGÖK¹, Nadire ESER¹, Furkan Toksözlü¹, Duygun Altýntaþ Aykan^1
1Kahramanmaras Sutcu Imam University, School of Medicine, Department of Pharmacology, Kahramanmaras
²Kahramanmaras Sutcu Imam University, School of Medicine, Department of Biochemistry, Kahramanmaras

Background: Skeletal muscle ischemia-reperfusion injury (IRI) is a major concern in various clinical settings and oxidative stress and neutrophil infiltration play a central role in this phenomenon. Unfortunately, clinicians have very few medicinal product options for both prevention and treatment of IRI. The aims of the present study were to examine the effects of epigallocatechin gallate (EGCG) on (1) skeletal muscle injury, (2) oxidative stress, and (3) neutrophil infiltration-related markers.
Methods: This was an experimental study conducted on rats. The IRI model utilizing an elastic rubber band was 4 + 2 h (IRI group). In sham-control (SC) group, all steps but band binding were preserved. Saline (10 mL/kg/i.p.) and EGCG (25 and 50 mg/kg/i.p.) were administered 30 minutes before the reperfusion (IRI-SF, IRI-25, and IRI-50 groups). Creatine phosphokinase (CPK) (primary end-point), lactate dehydrogenase (LDH), total oxidant status (TOS), total antioxidant status (TAS), myeloperoxidase (MPO), E-selectin, P-selectin, L-selectin, intercellular adhesion molecule-1 (ICAM-1), and various cytokines (IL-1β, IL-6, TNF-α) were determined in serum and gastrocnemius muscle samples.
Results: CPK, LDH, and TOS levels were higher in the IRI group compared to the SC group (P=0.001; P=0.0001; P=0.005); decreases, though not statistically significant, were observed in the IRI-50 group compared to the IRI group (P=0.628; P=0.167; P=0.444). Regarding TAS, a visible decrease was seen in the IRI compared to SC group (P=0.054), which was significantly increased by 50 mg/kg of EGCG (P=0.011). Regarding remaining parameters (except for IL-6), there were no statistically significant increases in IRI compared to SC and decreases in the EGCG-treated versus IRI groups.
Conclusion: We claim that EGCG possesses antioxidant activity. A beneficial effect stemming from its interaction with neutrophil infiltration-related markers, however, would only be suggestive.

Keywords: Reperfusion Injury, Skeletal Muscle, Epigallocatechin Gallate, Oxidative Stress, Neutrophil Infiltration, Cytokines

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Epigallocatechin Gallatýn Ýskemi-reperfüzyon Hasarý Üzerindeki Etkileri: Sýçanlarda Deneysel Bir Çalýþma

Yusuf Ergün¹, Yasarcan Baykisi², Metin Kilinc², AYÞE NUR MAVÝGÖK¹, Nadire ESER¹, Furkan Toksözlü¹, Duygun Altýntaþ Aykan^1
1Kahramanmaraþ Sütçü Ýmam Üniversitesi Týp Fakültesi Týbbi Farmakoloji Ana Bilim Dalý, Kahramanmaraþ
²Kahramanmaraþ Sütçü Ýmam Üniversitesi Týp Fakültesi Biyokimya Anabilim Dalý, Kahramanmaraþ

Amaç: Ýskelet kasý iskemi-reperfüzyon hasarý (ÝRH), çeþitli klinik durumlarda önemli bir endiþe kaynaðýdýr ve oksidatif stres ve nötrofil infiltrasyonu bu olguda merkezi bir rol oynar. Ne yazýk ki, klinisyenlerin hem ÝRH'nin önlenmesi hem de tedavisi için çok az týbbi ürün seçeneði vardýr. Mevcut çalýþmanýn amaçlarý, epigallocatechin gallatenin (EGCG) (1) iskelet kasý hasarý, (2) oksidatif stres ve (3) nötrofil infiltrasyonuyla iliþkili belirteçler üzerindeki etkilerini incelemektir.
Gereç ve yöntem: Bu, sýçanlar üzerinde yapýlan deneysel bir çalýþmadýr. Elastik bir lastik bant kullanan ÝRH modeli 4 + 2 saatti (ÝRH grubu). Sahte kontrol (SC) grubunda, bant baðlamasý hariç tüm adýmlar korundu. Serum fizyolojik (10 mL/kg/i.p.) ve EGCG (25 ve 50 mg/kg/i.p.), reperfüzyondan 30 dakika önce uygulandý (ÝRH-SF, ÝRH-25 ve ÝRH-50 gruplarý). Kreatin fosfokinaz (CPK) (primer uç-nokta), laktat dehidrogenaz (LDH), toplam oksidan durum (TOS), toplam antioksidan durum (TAS), miyeloperoksidaz (MPO), E-selectin, P-selectin, L-selectin, hücreler arasý adhezyon molekülü-1 (ICAM-1) ve çeþitli sitokinler (IL-1β, IL-6, TNF-α) serum ve gastrocnemius kas örneklerinde ölçüldü.
Bulgular: CPK, LDH ve TOS düzeyleri ÝRH grubunda SC grubuna göre daha yüksekti (P=0.001; P=0.0001; P=0.005); ÝRH-50 grubunda ÝRH grubuna göre istatistiksel olarak anlamlý olmayan düþüþler gözlendi (P=0.628; P=0.167; P=0.444). TAS açýsýndan, ÝRH’de SC grubuna göre görünür bir düþüþ görüldü (P=0.054) ve bu, 50 mg/kg EGCG ile önemli ölçüde arttý (P=0.011). Geri kalan parametreler açýsýndan (ÝL-6 hariç), ÝRH'de SC'ye göre istatistiksel olarak önemsiz artýþlar ve EGCG ile tedavi edilen gruplarda ÝRH gruplarýna göre düþüþler tespit edildi.

Anahtar Kelimeler: Reperfüzyon Hasarý, Ýskelet Kasý, Epigallocatechin gallate, Oksidatif Stres, Nötrofil Ýnfiltrasyonu, Sitokinler

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