The effect of chrysin on ischemia-reperfusion injury in the rat epigastric artery skin island flap

BACKGROUND: The return of oxygenated blood by reperfusion after ischemia triggers paradoxical tissue damage known as ischemia-reperfusion (I/R) injury. This study aims to investigate the effect of chrysin (C) on I/R injury in epigastric island flaps in rats.
METHODS: Thirty male Wistar albino rats were randomly divided into five groups (n=6): the sham control group (Group I), the flap I/R-untreated group (Group II), the I/R + C 10 mg/kg/day group (Group III), the I/R + C 50 mg/kg/day group (Group IV), and the I/R + C 100 mg/kg/day (Group V). Chrysin was administered orally for seven days before and after surgery. Flap elevation was performed on the eighth day, followed by eight hours of induced ischemia. Flap survival rate, as well as biochemical and histopathological parameters, were evaluated.
RESULTS: Oral administration of chrysin significantly reduced fibroblast activity at all treatment doses in rats subjected to I/R injury. Although improvements were observed in flap survival rate, oxidative stress index (OSI), and vascular proliferation at 10 mg/kg/day and 50 mg/kg/day doses, as well as in total oxidant status (TOS), tumor necrosis factor-alpha (TNF-α), active inflammation, chronic inflammation and ulceration across all treatment doses, these changes did not reach statistical significance. Total antioxidant capacity (TAC) values, consistent with existing literature, did not appear to influence the positive outcomes.
CONCLUSION: This study examined the effects of chrysin treatment on ischemia-reperfusion injury using a rat inferior epigastric artery skin island flap model. Although favorable molecular changes were observed, these did not translate into significant improvements in clinical outcomes.

Chrysin'in sýçan inferior epigastrik arter cilt ada flebinde iskemi reperfüzyon hasarýna etkisi

AMAÇ: Ýskemi sonrasý reperfüzyon ile oksijenize kanýn dokuya geri dönüþü, iskemi-reperfüzyon (Ý/R) hasarý olarak adlandýrýlan paradoksal doku yýkýmýný artýracak reaksiyonlarý baþlatýr. Çalýþmamýzýn amacý sýçan inferior epigastrik arter cilt ada flep modelinde chrysin'in(C) Ý/R hasarý üzerine etkisini ortaya koymaktýr.
GEREÇ VE YÖNTEM: 30 erkek Wistar albino sýçan randomize beþ gruba ayrýldý (n=6): sham-kontrol grubu (grup I), flep I/R ile tedavi edilmemiþ (grup II) grup, I/R-C-10 mg/kg/gün grubu (grup III), I/R-C-50 mg/kg/gün grubu (grup IV) ve I/R-C-100 mg/kg/gün grubu (grup V). Chrysin preoperatif ve postoperatif yedi gün boyunca oral olarak uygulandý. Sekizinci günde flepler eleve edildi ve denekler sekiz saat boyunca iskemiye maruz býrakýldý. Flep saðkalým oraný, biyokimyasal ve histopatolojik parametreler deðerlendirildi.
BULGULAR: Oral chrysin uygulamasý, I/R hasarýna maruz býrakýlan sýçanlarda tüm tedavi dozlarýnda fibroblast aktivitesini önemli ölçüde baskýlamýþtýr. Flep saðkalým oraný, oksidatif stres indeksi (OSÝ) ve vasküler proliferasyon parametrelerinde 10 mg/kg/gün ve 50 mg/kg/gün dozlarýnda, total oksidan stres (TOS), tümör nekroz faktörü-alfa (TNF-α), aktif inflamasyon, kronik inflamasyon ve ülserasyon parametrelerinde ise tüm tedavi dozlarýnda olumlu deðiþiklikler gözlense de bu deðiþiklikler istatistiksel anlamlýlýða ulaþmamýþtýr. Total antioksidan kapasite (TAK) deðerlerinin, mevcut literatürle tutarlý olarak, olumlu sonucu etkilemediði görülmüþtür.
SONUÇ: Çalýþmamýz, sýçan inferior epigastrik arter cilt adasý flebinde iskemi-reperfüzyon (I/R) hasarýna karþý chrysin tedavisinin etkilerini araþtýrmýþtýr. Moleküler düzeyde olumlu deðiþiklikler tespit edilmekle birlikte, bu bulgularýn klinik sonuçlara yansýmadýðý gözlemlenmiþtir.