Sýçanlarda ozonun baðýrsak iskemi-reperfüzyon hasarýnýn akut evresi üzerine etkisi

AMAÇ: Klinik uygulamalara benzer þekilde sýçanlarda ozonun intestinal iskemi-reperfüzyon (I/R) hasarýnýn akut fazý üzerindeki terapötik etkilerini incelemeyi amaçladýk.
GEREÇ VE YÖNTEM: On sekiz Wistar albino sýçaný kontrol (CG, n=6), sham (SG, n=6) ve ozon gruplarýna (OG, n=6) ayrýldý. Laparatomiyi takiben SG ve OG’de süperiyor mezenterik arter (SMA) 0/0 katgut dikiþle oklüde edildi, ancak CG’de herhangi bir giriþim yapýlmadan insizyon kapatýldý. Eþit derecede baðýrsak iskemisi elde etmek için bir doku oksijenasyon monitörü kullanýldý. Ýnsizyon kapatýldý ve OG’de, reperfüzyondan 20 dakika önce ozon/oksijen karýþýmý (0.7 mg/kg) intraperitoneal olarak enjekte edildi. SG ve OG’de 60 dakikalýk iskemi sonrasý cerrahi insizyon tekrar açýldý ve reperfüzyon saðlandý. Atmýþ dakikalýk reperfüzyondan sonra tüm gruplarda baðýrsak mukozal hasarýnýn (Chiu skoru) histopatolojik deðerlendirmesi ve oksidatif stres belirteçlerinin (nitrik oksit: NO, malonildialdehit: MDA, süperoksit dismutaz: SOD) biyokimyasal deðerlendirmesi için 2 cm’lik ince baðýrsak segmenti örneklendi.
BULGULAR: Chiu skorlarý SG ve OG’de CG’ye göre istatistiksel olarak yüksekti (sýrasýyla, p=0.002 ve p=0.002). Chiu skoru OG’de SG’ye göre daha yüksekti, ancak istatistiksel olarak anlamlý deðildi (p=0.175). MDA düzeyleri SG ve OG’de CG’ye göre istatistiksel olarak daha yüksekti (sýrasýyla p=0.004 ve p=0.010). Ancak, SG ve OG arasýndaki fark istatistiksel olarak anlamlý deðildi (p=0.522). SOD ve NO düzeyleri açýsýndan gruplar arasýnda anlamlý olarak fark yoktu (sýrasýyla, p=0.451 ve p=0.056).
TARTIÞMA: Literatürün aksine, tek doz ozon tedavisi, sýçanlarda baðýrsak I/R hasarýnda oksidatif stresi azaltmadý ve iskemik hasarý iyileþtirmedi. Potansiyel bir klinik kullaným için farklý zaman aralýklarýnda farklý dozlarý içeren daha ileri deðerlendirmeler yapýlmasý gerekmektedir.

The effects of ozone on the acute phase of intestinal ischemia-reperfusion injury in rats

BACKGROUND: In this study, we aimed to examine the therapeutic effects of ozone on the acute phase of intestinal ischemia-reperfusion (I/R) injury in rats to resemble clinical practice.
METHODS: Eighteen Wistar albino rats were assigned to control (CG, n=6), sham (SG, n=6) and ozone groups (OG, n=6). A midline laparotomy was performed and a superior mesenteric artery (SMA) in the SG and OG was occluded with a 0/0 catgut suture, but in the CG, the incision was closed without any intervention. Tissue oxygenation was monitored with a tissue oxygenation monitor to achieve the same grade during intestinal ischemia. The incision was closed and, in the OG, ozone/oxygen mixture (0.7 mg/kg) was injected intraperitoneally, 20 minutes before reperfusion. Surgical incision was reopened and reperfusion was achieved after 60 minutes of ischemia in the SG and OG. After 60 minutes of reperfusion, 2 cm small intestine segment was sampled for histopathological assessment of the intestinal mucosal damage (Chiu score) and biochemical assessment of oxidative stress markers (nitric oxide: NO, malondialdehyde: MDA, superoxide dismutase: SOD) in all groups.
RESULTS: The Chiu scores of the SG and OG were statistically increased than that of the CG (p=0.002; and p=0.002, respectively). Chiu score in the OG was higher compared to that in the SG, but not statistically significant (p=0.175). MDA levels were statistically higher in the SG and OG than that of the CG (p=0.004; and p=0.010, respectively). However, the difference between the SG and OG was not statistically significant (p=0.522). SOD and NO levels were not significantly different between groups (p=0.451 and p=0.056, respectively).
CONCLUSION: Contrary to the literature, single-dose ozone therapy did not reduce the oxidative stress or improve the ischemic damage in intestinal I/R injury in rats. Further evaluation with different doses in different time periods is needed for potential clinical use.