Ulus Travma Acil Cerrahi Derg. 2007; 13(4): 274-280

Effects of resveratrol on skeletal muscle in ischemia-reperfusion injury

Nurzat Elmalý¹, Ýrfan Esenkaya¹, Neþe Karadað², Ferhat Taþ¹, Nevzat Elmalý^3
1Department Of Orthopaedics And Traumatology, Medicine Faculty Of Inonu University, Malatya, Turkey
²Departments Of Pathology, Medicine Faculty Of Inonu University, Malatya, Turkey
³35th Department Of Surgery, Okmeydaný State Hospital, Ýstanbul, Turkey

BACKGROUND: Resveratrol, a polyphenol found in grape and red wine, was previously shown to have free radical scavenging and antioxidant properties in various tissues. In this study, the effects of resveratrol were investigated in muscle tissue concerning the ischemia reperfusion (I/R) injury of rat hindlimb. METHODS: Arterial circulation of right hindlimbs of 24 Sprague-Dawley rats was ceased by a tourniquet applied for four hours (h). The tourniquet was released at the end of 4th hours and rats were divided into four groups of six rats. Then, extremity was reperfused for 4h in group I and for 8h in group II. Resveratrol in 0.5% ethyl alcohol was administered with a dose of 10 mg/kg in the treatment groups (group I and group II) intraperitoneally. Only 0.5% ethyl alcohol were administered in the control groups (group III and group IV) intraperitoneally. Gastrocnemius muscle was used for histological assessments and the anterior tibial muscle was used for measurement of malondialdehyde (MDA) levels. RESULTS: MN infiltration, edema, changes in diameters of muscle fibers and segmental necrosis were less prominent in rats treated with resveratrol compared with control groups (p<0.05). The MDA levels was significantly lower in treatment groups (p<0.05). CONCLUSION: The results suggest that resveratrol may protect the skeletal muscles against I/R injury with its potent antioxidant properties.

Keywords: Antioxidants, free radicals; ischemia; rats, Sprague-Dawley; reperfusion; resveratrol; skeletal muscle.

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Ýskemi reperfüzyon yaralanmasýnda resveratrol’ün iskelet kasý üzerine etkileri

Nurzat Elmalý¹, Ýrfan Esenkaya¹, Neþe Karadað², Ferhat Taþ¹, Nevzat Elmalý^3
1Ýnönü Üniversitesi, Týp Fakültesi, Ortopedi Ve Travmatoloji Anabilim Dalý, Malatya
²Ýnönü Üniversitesi, Týp Fakültesi, Patoloji Anabilim Dalý, Malatya
³Saðlýk Bakanlýðý Okmeydaný Hastanesi, 5. Cerrahi Kliniði, Ýstanbul

AMAÇ: Üzümde ve kýrmýzý þarapta bulunan polifenol yapýdaki resveratrol’ün çeþitli dokularda serbest radikal temizleyicisi ve antioksidan özelliklere sahip olduðu önceki çalýþmalarda gösterilmiþtir. Bu yazýda sýçanlarýn arka ekstremitelerinde oluþturulan iskemi reperfüzyon (I/R) yaralanmasýnda resveratrol’ün kas dokusundaki etkileri araþtýrýldý.
GEREÇ-YÖNTEM: Yirmi dört adet Sprague-Dawley cinsi sýçanýn sað arka ekstremitelerine turnikenin uygulanmasý ile arteryel kan dolaþýmý 4 saat süreyle durduruldu. Dördüncü saatin sonunda turnike serbestleþtirilerek sýçanlar her biri 6 adetten oluþan 4 gruba ayrýldý. Ekstremitenin grup 1’de 4 saat, grup 2’de 8 saat süreyle yeniden perfüzyonuna izin verildi. Tedavi gruplarýna (grup 1 ve grup 2) %0,5 etil alkol içinde 10 mg/kg resveratrol intraperitoneal (i.p.) uygulandý. Ayný sürelerle reperfüzyonuna izin verilen kontrol gruplarýna (grup 3 ve grup 4) ise sadece %0,5 etil alkol i.p. uygulandý. Histolojik deðerlendirme için gastroknemius kasý ve malondialdehit (MDA) seviyelerinin ölçümü için tibialis anterior kasý çýkarýldý.
BULGULAR: Resveratrol ile tedavi edilen gruplarda kas dokusunda polimorf çekirdekli lökosit infiltrasyonu, ödem, kas liflerinin boyutlarýnda deðiþiklik ve segmental nekroz kontrollerle karþýlaþtýrýldýðýnda belirgin azalmýþtý (p<0.05). MDA seviyeleri de ayný þekilde tedavi grubunda düþüktü (p<0.05). SONUÇ: Bu sonuçlar resveratrolün güçlü antioksidan etkisi ile iskelet kasýný I/R yaralanmasýna karþý koruyabileceðini düþündürmektedir.

Anahtar Kelimeler: Antioksidanlar, serbest radikaller; iskemi; sýçan, Sprague-Dawley cinsi; reperfüzyon; resveratrol; iskelet kasý.

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