Ulus Travma Acil Cerrahi Derg. 2008; 14(2): 87-95

Repair of critical bone defects with injectable platelet rich plasma/bone marrow-derived stromal cells composite: experimental study in rabbits

Xiaobing Cheng¹, Delin Lei¹, Tianqiu Mao¹, Shuyong Yang¹, Fulin Chen², Wei Wu^1
1Department Of Oral And Maxillofacial Surgery, College Of Stomatology, Xi’an, Prc
²Faculty Of Life Science, Northwest University, Xi’an, Prc

BACKGROUND
Platelet-rich plasma (PRP) has been applied to promote bone healing and developed as a novel material for bone regeneration. This study aimed to investigate the feasibility of PRP carrier to deliver bone marrow derived stromal cells (BMSCs) and regenerate bone tissues to reconstruct critical bone defects in rabbits.
METHODS
Critical sized defect were made on eighteen rabbits’ crania and treated by different composites: BMSCs/PRP (n=6); Autogenous particulate cancellous bone group (n=6) and PRP alone group (n=6). The defects were evaluated by gross observation, radiographic examination, histological examination, and mechanical examination at 12 weeks postoperatively.
RESULTS
The results showed that repair of bone defect was the least in PRP alone group, and significant new bone formation could be observed in BMSCs/PRP group and particulate cancellous bone group, radiopacity area in BMSCs/PRP group attained 76.5%, which was in the same range of that in autogenous particulate cancellous bone group (82.4% in radiopacity area), compressive strength of engineered bone in BMSCs/PRP group attained 71% of that in autogenous particulate cancellous bone group (p<0.05).
CONCLUSION
These data implicated that BMSCs delivered from PRP gel can repair bony defect in immunocompetent animals, and the tissue engineered bone in BMSCs/PRP group is comparable to autogenous particulate cancellous bone group for the repair of critical-sized bone defect.

Keywords: Bone marrow-derived stromal cells, bone regeneration; injectable hydrogel; platelet rich plasma; rabbits; tissue engineering/ methods.

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Kritik kemik hasarlarýnda trombositce zengin plazma/kemik iliði kaynaklý stromal hücrelerin kompozitle enjekte edilerek tamir edilmesi: Tavþanlarda deneysel çalýþma

Xiaobing Cheng¹, Delin Lei¹, Tianqiu Mao¹, Shuyong Yang¹, Fulin Chen², Wei Wu^1
1Dördüncü Askeri Týp Üniversitesi, Stomatoloji Koleji, Aðýz Ve Diþ Cerrahisi Anabilim Dalý, Xi’an, Çin Halk Cumhuriyeti
²Kuzeybatý Üniversitesi, Yaþam Bilimi Fakültesi, Xi’an, Çin Halk Cumhuriyeti

AMAÇ
Trombositce zengin plazma (TZP), kemik iyileþmesine katkýda bulunmak üzere uygulanmýþ ve kemik yenilenmesine yönelik yeni bir materyal olarak geliþtirilmiþtir. Bu çalýþmada tavþanlarda, kemik iliði kaynaklý stromal hücreler (KÝKSH) vermek ve kritik kemik iliði hasarlarýný düzeltmek için kemik dokularýný yenilemek üzere TZP taþýyýcýlýðýnýn fizibilitesinin incelenmesi amaçlandý.
GEREÇ VE YÖNTEM
On sekiz tavþanýn kafatasýna ciddi ölçüde hasar verildi ve bunlar deðiþik kompozitlerle tedavi edildi: KÝKSH/TZP (n=6); otojen partiküler kansellöz kemik grubu (n=6) ve tek baþýna TZP grubu (n=6). Hasarlar, ameliyattan sonra 12. haftada gros gözlem, radyografik, histolojik ve mekanik incelemelerle deðerlendirildi.
BULGULAR
Kemik hasarý tamirinin tek baþýna TZP grubunda en az düzeyde olduðunu, KÝKSH/TZP grubu ile partiküler kansellöz kemik grubunda anlamlý yeni kemik oluþumu gözlenebileceðini, radyoopasite alanýnýn KÝKSH/TZP grubunda otojen partiküler kansellöz kemik grubundakinin (radyoopasite alanýnda %82.4) %76.5’i oranýnda kazanýldýðýný, planlanarak düzenlenmiþ kemiðin kompresif gücünün KÝKSH/TZP grubunda otojen partiküler kansellöz kemik grubundakinin %71’i oranýnda kazanýldýðýný gösterdi (p<0.05).
SONUÇ
TZP jelden salýverilen KÝKSH’lerin, baðýþýklýk sistemi normal olan hayvanlarda kemik hasarýný tamir edebileceðini ve KÝKSH/TZP grubundaki planlanarak düzenlenmiþ kemiðin kritik ölçüdeki hasarýnýn tamirinde otojen partiküler kansellöz kemik grubundaki ile karþýlaþtýrýlabilir olduðunu göstermiþtir.

Anahtar Kelimeler: Kemik iliði kaynaklý stromal hücreler, kemik yenilenmesi; þýrýnga edilebilir hidrojel; tavþanlar; trombositce zengin plazma; doku mühendisliði/yöntemler.

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