Role of Alvarado score and biological indicators of C-reactive protein, procalicitonin and neopterin in diagnosis of acute appendicitis

BACKGROUND: The determination of a definitive preoperative diagnosis of acute appendicitis (AA) remains a challenge; however, delays in diagnosis increase complication rates. The aim of this study was to investigate the contribution of the Alvarado score (AS) alone and the AS combined with the use of the biological indicators of C-reactive protein (CRP), procalcitonin (PCT) and neopterin (NP) in the diagnosis.
METHODS: Serum was collected from 100 patients who were admitted to the general surgery clinic of Istanbul University, Cerrahpasa Medical Faculty between March 4, 2014 and July 29, 2015 with the pre-diagnosis of AA and who agreed to take part in the study. The serum samples were stored at -70°C. The patients were divided into 2 groups: AA-positive (n=60) and AA-negative (n=40). The AA positive group was divided into subgroups of complicated (n=11), uncomplicated AA (n=49) and the AS, CRP, PCT, NP levels were compared.
RESULTS: The study population consisted of 45 men (45%) and 55 women (55%), with a mean age of 32.8±13.7 years (range: 18–92 years). There was no significant difference between the groups in age and gender. There were 24 patients with an AS ≤4 (3 had surgery), 35 patients with an AS of 5–7 (22 had surgery), and 41 patients with an AS of 8–10 (38 had surgery). Three of the 63 patients who underwent surgery were diagnosed with a normal appendix. The serum CRP, PCT, and NP measures were found to be inadequate to make an AA diagnosis alone, these values increased the sensitivity and specificity of the AS. The biological indicators were also significant in differentiating between the complicated and uncomplicated AA groups (p<0.05).
CONCLUSION: Although the AS is useful, additional testing and clinical approaches are valuable to inform the diagnostic procedure. When considered alone, serum CRP, PCT and NP values are insufficient for a diagnosis of AA. However, they increased the diagnostic value of the AS and can be helpful in distinguishing complicated AA cases.

Akut apandisit tanýsýnda Alvarado skorlamasý, C-reaktif protein, prokalsitonin ve neopterin biyolojik belirteçlerin yeri

AMAÇ: Akut apandisit (AA), günümüzde hala kesin tanýsýný koymak mümkün deðildir. Tanýdaki gecikmeler, komplikasyon oranlarýný artýrmaktadýr. Alvarado skorlamasý (AS) ve biyolojik belirteçlerin (C-reaktif protein [CRP], prokalsitonin [PCT], neopterin [NP]) tek ve AS ile birlikte kullanýlmalarýnýn tanýya olan katkýlarýnýn araþtýrýlmasý amaçlandý.
GEREÇ VE YÖNTEM: Ýstanbul Üniversitesi Cerrahpaþa Týp Fakültesi, Genel Cerrahi Kliniði’ne, 04.03.2014–29.07.2015 tarihleri arasýnda, AA ön tanýsýyla, gönüllü 100 hastadan, yatýþ öncesi serumlarý alýnarak -70 derecede saklandý. Hastalar, AA pozitif (n=60), negatif (n=40) gruplandý. AA pozitif grup; komplike (n=11), nonkomplike (n=49) olarak gruplandýrýlarak, AS, CRP, PCT ve NP deðerleri karþýlaþtýrýldý.
BULGULAR: Çalýþmaya alýnan hastalarýn; 45’i erkek (%45), 55’i kadýn (%55),yaþ ortalamalarý 32.8±13.7 18–92) yýl idi. Gruplar arasýnda, yaþ, cinsiyet açýsýndan anlamlý fark yoktu. AS daðýlýmý; AS ≤4 (n=24) üçü, AS 5–7 (no: 35) 22’si, AS 8–10 (no: 41) 38’i ameliyat edildi. Ameliyat edilen 63 hastanýn üçünde normal apendiks saptandý. AA pozitif ve negatif gruplarýn, serum CRP, PCT, NP deðerlerinin tanýda yetersiz olduðu ancak AS’nin sensivite ve duyarlýlýðýný artýrdýðý görüldü. Biyolojik belirteçlerin ortalamasý karþýlaþtýrýldýðýnda; komplike AA grup ayrýmýnda anlamlýydý (p<0.05).
TARTIÞMA: Alvarado skorlamasý, iyi bir skorlama olmasýna karþýn, ek inceleme ve klinik yaklaþýmlara ihtiyaç vardýr. Serum CRP, PCT ve NP deðerleri, tek baþlarýna, AA tanýsýnda yetersizdir. Ancak AS’nin deðerini arttýrmakta ve komplike AA olgularýný ayýrmada yararlýdýr.