EFFECTS OF PREDNISOLONE AND DEXAMETHASONE ON ALKALI BURNS OF THE ESOPHAGUS IN RATS

In this experimental study, we aimed to evaluate the effects of prednisolone and dexamethasone developing of strictures in alkali esophageal burns of rats. Fourty Spraque-Dawley rats weighting between 225 and 250 gram were used. Rats were randomly divided into 4 groups. In Group 1 rats (control), esophageal lumen was washed with normal saline. In Group 2 rats, distal esophagus was burned with 50% NaOH solution and parenteral antibiotics were given. In Group 3 rats, after esophageal burn developing, dexamethasone (1 mg/kg/day) and parenteral antibiotics were given. In Group 4 rats, after esophageal burn developing, prednisolone (2mg/kg/day) and parenteral antibiotics were given. All rats were sacrificed at 21 st days of procedure. All rats were evaluated for submucosal collagen increase damage of muscularis mucosa and tunica muscularis histopathologically. There were nosubmucosal collagen increase, muscularis mucosa and tunica muscularis damage in Group 1. There was significant difference for submucosal collagen increase, muscularis mucosa and tunica muscularis damage when groups 2,3 and 4 was compared to Group I (p<0.0001, p<0.0001, p<0.0001). There was also significant difference for 3 parameter when Group 3 was compared to Group 2(p<0.001,p<0.001,p<0.001).There was significant damages in muscularis mucosa and tunica muscularis when Group 4 was compared to Group 2 (p< 0.001, p < 0.001). No significant difference was found for submucosal collogene developing between when Group 4 was compared to Group 2 (p>0.05). As conclusion, prednisolone may reduce submucosal collagen synthesis, but the preventine effects ofesophageal stricture is less than dexamethasone.

KATLARDA ALKALÝ ÖZOFAGUS YANIÐI ÜZERÝNE DEKSAMETAZON VE PREDNÝZOLONUN ETKÝLERÝ

Çalýþmamýzda sýçanlarda alkali ile oluþturulmuþ özofagus yanýðýnda deksametazon ve prednizolonun striktür geliþimi üzerine etkilerinin karþýlaþtýrýlmasý amaçlanmýþtýr. Çalýþmamýzda 40 adet Spraque-Dawley cinsi rat (225-250 gr) kullanýldý. Ratlar herbiri 10 denekten oluþan 4 gruba ayrýldý. Grup 1 ratlarda özofageal lümen içi normal serum fizyolojik ile yýkandý. Grup 2 sýçanlarda %50'lik NaOH ile özofagus yanýðý oluþturuldu ve parenteral antibiyotik verildi. Grup 3 ratlarda özofagus yanýðýný takiben deksametazon (1mg/kg/gün)+parenteral antibiyotik baþlandý. Grup 4 ratlarda özofagus yanýðýný takiben prednizolon (2mg/kg/gün) +parenteral antibiyotik baþlandý. Ratlarýn tümü 21. günde sakrifiye edilerek abdominal özefaguslarý histopatolojik inceleme için çýkarýldý. Grup 1 rotlarda submukozal kollojende artýþ, muskularis mukoza hasarý ve tunika muskularis hasarýna rastlanmadý. Grup 2,3 ve 4 ratlarýn, Grup 1 ratlarla karþýlaþtýrýlmasýnda submukozal kollajende artýþ, muskularis mukoza hasarý ve tunika muskularis hasarý saptandý (p<0,0001, p<0,000l, p<0,0001. Grup 3 rotlar ile grup 2 ratlarýn her üç parametre açýsýndan karþýlaþtýrýlmasýnda grup 3'de lezyonlarýn daha az geliþtiði ve istatistiksel olarak anlamlý olduðu saptandý (p < 0,001, p<0,001, p<0,001). Ayný paremetrelerin ýþýðýnda Grup 4 ile Grup 2 ratlarýn karþýlaþtýrýlmasýnda muskularis mukoza ve tunika muskularis hasarý açýsýndan anlamlý farklýlýk saptandý (p < 0,001, p < 0,001). Grup2 ile Grup 4 arasýnda submukozal kollojende artýþ yönünden fark bulunmadý (p > 0,05). Sonuç olarak, prednizolon muskularis mukozaya kadar ilerleyen yeni kollajen sentezini azaltabilir ancak darlýk oluþumunu azaltýcý etkisi deksametazona göre belirgin deðildir.