Sýçanlarda karaciðer laserasyon modelinde Ankaferd Blood Stopper ve fibrin yapýþtýrýcýnýn hemostatik etkinliðinin karþýlaþtýrýlmasý

AMAÇ
Ankaferd Blood Stopper® (ABS) son yýllarda kullanýma giren, organik kökenli topikal bir hemostatik ajandýr. Bu çalýþmanýn amacý, sýçanlarda karaciðer laserasyon modelinde halen yaygýn klinik kullanýmda olan fibrin yapýþtýrýcý (FY) ile ABS’nin etkinliði karþýlaþtýrmaktýr.
GEREÇ VE YÖNTEM
Wistar Albino cinsi 32 adet sýçan eþit olarak rastgele dört gruba ayrýldý. Sham grubunda (Grup 1) karaciðer sadece eksplore edildi. Çalýþma gruplarýnda (Grup 2, 3, 4) karaciðer sol lob ön yüzünde 1 cm uzunluðunda 2 mm derinliðinde 3 adet laserasyon oluþturuldu. Grup 2 ve 3’de sýrasýyla ABS ve FY topikal hemostatik olarak kullanýldý ve kanama kontrol altýna alýndý. Kontrol grubunda (Grup 4) hiçbir materyal kullanýlmadý. Kanama zamaný, hematokrit düzeylerindeki deðiþiklikler, karýn içi yapýþýklýk düzeyi ve histopatolojik etkileri deðerlendirildi.
BULGULAR
ABS ve FY gruplarýnda topikal hemostaz zamaný arasýnda anlamlý fark bulunmazken, kontrol grubunda bu süre belirgin olarak daha uzundu, sýrasýyla 17 (15-20) sn, 18 (16-20) sn, 70 (64-74) sn (p<0,05). Karýn içi yapýþýklýk deðerlendirmesinde çalýþma gruplarý arasýnda belirgin farklýlýk görülmedi, Grup 1: 1 (0-1), Grup 2: 2 (1-3), Grup 3: 2 (1-3), Grup 4: 2 (1-3) (p>0,05). Mikroskobik incelemede ABS ve FY’nin karaciðer ve çevre dokulardaki histopatolojik etkilerinin benzer olduðu görüldü (p>0,05).
SONUÇ
ABS sýçanlarda karaciðer laserasyon modelinde FY ile eþit topikal hemostatik etkinliðe sahiptir. Karýn içi kullanýmda yapýþýklýk ve histopatolojik etkileri açýsýndan her iki materyal arasýnda anlamlý fark yoktur.

Comparison of the hemostatic efficiency of Ankaferd Blood Stopper and fibrin glue on a liver laceration model in rats

BACKGROUND
Ankaferd Blood Stopper® (ABS) is an organic topical hemostatic agent that has become available in recent years. The aim of this study was to compare the effectiveness of ABS in a liver laceration model in rats with that of fibrin glue (FG), which is currently being used widely in clinics.
METHODS
Thirty-two Wistar Albino type rats were randomly divided into four groups. In the Sham group (Group 1), only the liver was explored. In the other study groups (Groups 2, 3 and 4), three incisions were performed, each 1 cm long and 2 mm deep, on the front of the left lobe of the livers. In Groups 2 and 3, ABS and FG were used as hemostatic agents, respectively. No materials were used for the injuries in the Control Group (Group 4). Bleeding periods, changes in the hematocrit levels, intraabdominal adhesion levels, and histopathological effects were taken into consideration.
RESULTS
There was no significant difference between the period of hemostasis in Groups 2 and 3, whereas the same period was evidently longer in Group 4 (17 (15-20) sec, 18 (16-20) sec, 70 (64-74) seconds, respectively; p<0.05). No significant difference was detected between the groups regarding intraabdominal adhesion levels (Group 1: 1 (0-1), Group 2: 2 (1-3), Group 3: 2 (1-3), Group 4: 2 (1-3); p>0.05). Microscopic evaluations revealed similar histopathological effects of ABS and FG on the liver and surrounding tissues (p>0.05).
CONCLUSION
The topical hemostatic effectiveness of ABS was shown to be comparable to FG in a liver laceration model in rats. There was no significant difference between these materials regarding adhesion formation in intraabdominal use or histopathological effects.