L-ARGÝNÝN'ÝN NÖTROFÝLLER ÜZERÝNDEN POSTOPERATÝF ADEZYON OLUÞUMUNA ETKÝLERÝ

Cerrahi sonrasý oluþan intraperitoneal adezyonlar, infertilite, aðrý ve barsak obstrüksüyonuna neden olan önemli komplikasyonlardýr. Bu çalýþma L-arginin ve nötrofillerin postoperatif intraperitoneal adezyon oluþumundaki etkilerini araþtýrmak amacýyla yapýlmýþtýr. 16 sýçan iki eþit gruba ayrýldý; grup I (n=8) 5gün süreyle günlük tek doz % 0.9'luk izotonik sodyum klorür (salin), grup II (n=8) 5 gün süreyle günde tek doz L-arginin aldý ve uterin boynuz ve çekuma cerrahi hasar yapýldý. Adezyon indüklenmesinden sonraki 2 haftanýn sonunda adezyon skoru belirlendi. Adezyon dokusu histolojik ve biyokimyasal olarak incelendi. Adezyon oluþumunun ilk günü nötrofil sayýsý ve nötrofil adezivite indeksi tesbit edildi. Kontrol grubundaki adezyon geliþimi, L-arginin grubuna göre belirgin olarak daha az idi (p < 0.01). Kontrol grubu ile kýyaslandýðýnda, L-arginin grubunda adezif doku hidroksiprolin ve kollajen seviyeleri belirgin olarak artmýþtý (p < 0.05 ve p < 0.001). Sonuç olarak, bu hayvan modelinde, L-arginin postoperatif adezyonlarý azaltmada yararlý bulunmamýþtýr.

THE EFFECTS OF L-ARGININE ON POSTOPERATIVE PERITONEAL ADHESION FORMATION BY ITS EFFECTS ON NEUTROPHILS

Postoperative intraperitoneal adhesions are major postsurgical complications that cause infertility, pain, and intestinal obstruction. The aim of this study was to investigate the effects of L-arginine and neutrophils on the formation of postoperative intraperitoneal adhesion. Sixteen rats, equally divided into two groups; group I (n=8) received daily single dose saline for five days, group II (n=8) received daily single dose L-arginine for five days, and underwent surgical injury to the uterine horn and the cecum. The adhesion scores were determined at the end of the second week after adhesion induction. Adhesive tissue also histologically and biochemically evaluated. Neutrophil counts and neutrophil adhesivity index were determined in the first day of adhesion induction. Adhesion formation in the control group was significantly less than that of L-arginine group (p< 0.01). Hydroxyproline and collagen levels of adhesive tissue were significantly increased in the L-arginine group when compared with control group (p < 0.05 and p < 0.001). We concluded that L-arginine was not beneficial in reducing postoperative adhesions in this animal model.