Does ß-Glucan can have protective role against oxidative stress in experimental sepsis model in rats? [Ulus Travma Acil Cerrahi Derg]
Ulus Travma Acil Cerrahi Derg. Ahead of Print: UTD-79002 | DOI: 10.14744/tjtes.2019.79002  

Does ß-Glucan can have protective role against oxidative stress in experimental sepsis model in rats?

Meltem Demir1, KADER KÖSE2, Cevat YAZICI2, Erdoðan Mütevelli Sözüer3
1Department of Medical Biochemistry, Istinye University, Faculty of Medicine, Istanbul, Turkey.
2Department of Medical Biochemistry, Erciyes University, Faculty of Medicine Kayseri, Turkey
3Department of General Surgery, Erciyes University, Faculty of Medicine Kayseri, Turkey

Objective: Very high mortality rate in sepsis may be related to oxidative stress
This study was performed on the rats to investigate the presence of oxidative stress and also the potential protective effect of ß-glucan in the intra-abdominal sepsis model formed by cecal ligation-perforation (CLP).
Methods: Male 30 rats were equally divided into three groups as “Sham”, “Sepsis” and “ß-Glucan”. Only laparotomy was performed in the Sham group, and sepsis was induced by CLP in Sepsis and ß-Glucan groups. Following CLP, a single dose of 4 mg ß-glucan/kg was also intraperitoneally administered to β-Glucan group. Blood and tissue (liver, lung and kidney) samples were taken from Sepsis and ß-Glucan groups, after sepsis development determined at the end of the 48th hour, also from Sham group.
The levels of myeloperoxidase (MPO)and advanced oxidation protein products (AOPP) were determined in plasma samples; and the malondialdehyde (MDA) were measured in plasma and tissues.
Results: MPO and AOPP were higher in both the Sepsis and ß-Glucan groups; however plasma and tissue MDA levels were higher only in Sepsis group than the Sham group (p<0.05). However, when compared to the Sepsis group, all parameters measured, except kidney MDA, were significantly lower in the ß-Glucan group (p< 0.05).
Conclusion: In this first study to investigate the AOPP levels in CLP sepsis model; ROS produced by the reaction of MPO derived from neutrophils may form oxidative damage to the proteins, compared to the lipids, and ß-glucan may be used as an alternative agent for sepsis treatment.

Keywords: Sepsis, B-Glucan, Reactive oxygen species, Advanced oxidation protein products, Myeloperoxidase




Corresponding Author: Meltem Demir, Türkiye


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