Effects of sugammadex on ischemia reperfusion in a rat extremity model

BACKGROUND: The hypothesis of our study is that sugammadex has protective efficacy against ischemia-reperfusion (I/R) injury in rats.
METHODS: Our study included 28 male Wistar Albino rats. The rats were assigned to four groups. The sham group had no procedure other than anesthesia administration. The control group received three hours of ischemia and 24 hours of reperfusion. The Sgdx4 group received 4 mg/kg, and the Sgdx16 group received 16 mg/kg sugammadex intravenously, and then, reperfusion was applied. Histopathological investigation, and serum creatine kinase (CK), lactate dehydrogenase (LDH), and serum and tissue malondialdehyde (MDA) and superoxide dismutase (SOD) analyses were performed.
RESULTS: When the sham group and the control group were compared, there were statistically significant differences histopathologically (p<0.01). There was no significant difference between the Sgdx4 group compared with the sham and control groups histopathologically (p>0.01). There was a significant difference between the Sgdx16 group and the sham group histopathologically (p<0.01). There were significant differences between the sham and control groups concerning CK and LDH levels (p<0.01). There was a significant difference in the levels of CK between the control group and Sgdx4 group and in the levels of CK and LDH between the control group and Sgdx16 group (p<0.01).
CONCLUSION: In our study, we examined the histological and biochemical protective effects of 4 mg/kg sugammadex on unilateral lower extremity I/R injury in rats. The findings suggest that a 4 mg/kg dose of sugammadex was more effective than a 16 mg/kg dose.

Sýçan ekstremite modelinde iskemi reperfüzyon üzerine sugammadeks’in etkisi

AMAÇ: Çalýþmamýzýn hipotezi, sugammadeks’in sýçanlarda iskemi reperfüzyon (Ý/R) hasarýna karþý koruyucu etkinliðe sahip olmasýdýr.
GEREÇ VE YÖNTEM: Çalýþmaya 28 erkek Wistar Albino sýçaný dahil edildi. Sýçanlar dört gruba ayrýldý. Sham grubuna anestezi uygulamasý dýþýnda bir giriþim uygulanmadý. Kontrol grubuna 3 saat iskemi ve 24 saat reperfüzyon uygulandý. Sgdks4 grubu ve Sgdks 16 grubu, intravenöz olarak sýrasýyla 4 mg/kg ve 16 mg/kg sugammadeks aldý ve ardýndan reperfüzyon uygulandý. Histopatolojik inceleme ve serum kreatin kinaz (CK), laktat dehidrojenaz (LDH), serum ve doku malondialdehid (MDA) ve süperoksit dismutaz (SOD) analizleri yapýldý.
BULGULAR: Sham grubu ve kontrol grubu karþýlaþtýrýldýðýnda, histopatolojik olarak istatistiksel olarak anlamlý farklýlýklar vardý (p<0.01). Sgdks4 grubu ile sham ve kontrol gruplarý arasýnda histopatolojik olarak anlamlý fark yoktu (p>0.01). Sgdks16 grubu ile sham grubu arasýnda histopatolojik olarak anlamlý bir fark vardý (p<0.01). Sham ve kontrol gruplarý arasýnda CK ve LDH düzeyleri açýsýndan anlamlý fark vardý (p<0.01). Kontrol grubu ile Sgdks4 grubu arasýnda CK düzeyleri arasýnda ve kontrol grubu ile Sgdks16 grubu arasýnda CK ve LDH düzeylerinde anlamlý bir fark vardý (p<0.01).
TARTIÞMA: Çalýþmamýzda, sýçanlarda tek taraflý alt ekstremite Ý/R yaralanmasý üzerine 4 mg/kg sugammadeks’in histolojik ve biyokimyasal koruyucu etkileri incelendi. 4 mg/kg sugammadeks dozunun 16 mg/kg dozdan daha etkili olduðunu görüldü.