EFFECT OF TOCOPHEROL ON PATHOGENESIS OF GASTRIC STRESS ULCERATION AS A RADICAL SCAVENGER IN RATS

Recent studies have demonstrated that free oxygen radicals were involved in many pathologic processes and significant improvements were gained by using radical scavengers in numerous human diseases. The authors surveyed the pathogenic role of free oxygen radicals in gastric stress ulceration and evaluated the therapeutic potency of Tocopherol (Vitamin E) as a radical scavenger in the management of this disease. Forty female Wistar albino rats were randomized into four equal groups (n= 10). Nothing was done to group LTlie other three groups were stressed. Group 2 was not treated, group 3 was given ranitidine alone and group 4 was administered a combination of ranitidine and tocopherol (vitamin E). Twenty four hours later, all the animals were sacrificed and samples were taken for biochemical analysis, pathologic-anatomic examination and chemiluminescence measurements. No significant differences were found among the four groups in terms of the results of biochemical analysis except the glucose levels. Significantly lower ulcer indexes and chemiluminescence values were found in both Group 3 and 4 in regard to Group 2, but the difference between Group 2 and 3 was not significant in terms of the same values. Our study has demonsrated the ulcerogenic effects of free oxygen radicals but more detailed data are required for evaluation of the ameliorating potency of tocopherol as a radical scavenger in gastric stress ulceration.

GASTRÝK STRES ÜLSERÝ PATOGENEZÝNDE TOKOFEROLÜN ETKÝSÝ DENEYSEL ARAÞTIRMA

Yakýn zamanda yapýlan çalýþmalara göre serbest oksijen radikalleri birçok patolojik süreçte rol oynamaktadýrlar. Yazarla gastrik stres ülserlerinde serbest oksijen radikallerinin patojenik rolünü araþtýrmýþ ve tokoferolün (vitamin E) terapötik potansiyelini deðerlendirmiþlerdir. 40 diþi Wistar Albino rat dört eþit gruba ayrýlmýþtýr (n= 10).Grup 1 de hiçbir iþlem yapýlmamýþ, diðer gruplarda ise stress oluþturulmuþtur. Grup 2 tedavi edilmemiþ, Grup3'e sadece ranitidin verilmiþ, Grup 4'e ise ranitidin ve tokoferol (vitamin E) kombinasyonu uygulanmýþtýr. 24 saat sonra tüm hayvanlar sakrifiye edilmiþ, biyokimyasal analizler, patolojik inceleme ve chemilüminesan ölçümler için örnek alýnmýþtýr. Glukoz düzeyleri dýþýnda biyokimyasal analizler açýsýndan 4 grup arasýnda anlamlý fark bulunamamýþtýr. Grup 3 ve 4 'de grup 2 'ye göre anlamlý olarak düþük ülser indeksleri ve chemilüminesan deðerler bulunmuþ ancak ayný deðerler açýsýndan Grup 2 ve 3 arasýnda anlamlý bir fark bulunamamýþtýr. Bizim çalýþmamýz serbest oksijen radikallerinin ülserojenik etkilerini göstermiþtir ancak tokoferolün gastrik stres ülserinde tedavi edici etkisini göstermek için daha detaylý bilgilere ihtiyaç vardýr.